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LearnAM09354PU-N
antibody from Acris Antibodies GmbH
Targeting: EGLN1
C1orf12, HIFPH2, PHD2, SM-20, ZMYND6
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- AM09354PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09354PU-N, RRID:AB_10649296
- Product name
- anti EGLN1 / PHD2
- Antibody type
- Monoclonal
- Antigen
- Recombinant EGLN1 (271-426 aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT1E9
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EGLN1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EGLN1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
