Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP1-41081 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-41081, RRID:AB_2130804
- Product name
- Rabbit Polyclonal MLK4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references MLK3 phosphorylation by ERK1/2 is required for oxidative stress-induced invasion of colorectal cancer cells.
Osmotic and heat stress-dependent regulation of MLK4β and MLK3 by the CHIP E3 ligase in ovarian cancer cells.
Spinal 5-HT3 receptors mediate descending facilitation and contribute to behavioral hypersensitivity via a reciprocal neuron-glial signaling cascade.
Schroyer AL, Stimes NW, Abi Saab WF, Chadee DN
Oncogene 2018 Feb 22;37(8):1031-1040
Oncogene 2018 Feb 22;37(8):1031-1040
Osmotic and heat stress-dependent regulation of MLK4β and MLK3 by the CHIP E3 ligase in ovarian cancer cells.
Blessing NA, Kasturirangan S, Zink EM, Schroyer AL, Chadee DN
Cellular signalling 2017 Nov;39:66-73
Cellular signalling 2017 Nov;39:66-73
Spinal 5-HT3 receptors mediate descending facilitation and contribute to behavioral hypersensitivity via a reciprocal neuron-glial signaling cascade.
Guo W, Miyoshi K, Dubner R, Gu M, Li M, Liu J, Yang J, Zou S, Ren K, Noguchi K, Wei F
Molecular pain 2014 Jun 9;10:35
Molecular pain 2014 Jun 9;10:35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MLK4 Antibody [NBP1-41081] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit antiMLK4 antibody NBP1-41081 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MLK4 was also immunoprecipitated by rabbit anti-MLK4 antibody NBP1-41080, which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MLK4 Antibody [NBP1-41081] - Total MLK4 expression in breast cancer cells. Image from verified customer review.