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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- AF1494 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat TrkB Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) TrkB is observed, and less than 2% cross-reactivity with rhTrkA, recombinant rat TrkA, and recombinant mouse TrkC is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P15209- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA.
PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis.
Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice.
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration.
Functional Local Proprioceptive Feedback Circuits Initiate and Maintain Locomotor Recovery after Spinal Cord Injury.
Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines.
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells.
Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo.
Genetic Tracing of Ca(v)3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System.
Treadmill exercise suppressed stress-induced dendritic spine elimination in mouse barrel cortex and improved working memory via BDNF/TrkB pathway.
Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In Vivo.
Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway.
KCNQ Potassium Channels Modulate Sensitivity of Skin Down-hair (D-hair) Mechanoreceptors.
Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes.
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
Embryonic sympathoblasts transiently express TrkB in vivo and proliferate in response to brain-derived neurotrophic factor in vitro.
Desiderio S, Vermeiren S, Van Campenhout C, Kricha S, Malki E, Richts S, Fletcher EV, Vanwelden T, Schmidt BZ, Henningfeld KA, Pieler T, Woods CG, Nagy V, Verfaillie C, Bellefroid EJ
Cell reports 2019 Mar 26;26(13):3522-3536.e5
Cell reports 2019 Mar 26;26(13):3522-3536.e5
PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis.
Bartesaghi L, Wang Y, Fontanet P, Wanderoy S, Berger F, Wu H, Akkuratova N, Bouçanova F, Médard JJ, Petitpré C, Landy MA, Zhang MD, Harrer P, Stendel C, Stucka R, Dusl M, Kastriti ME, Croci L, Lai HC, Consalez GG, Pattyn A, Ernfors P, Senderek J, Adameyko I, Lallemend F, Hadjab S, Chrast R
Cell reports 2019 Mar 26;26(13):3484-3492.e4
Cell reports 2019 Mar 26;26(13):3484-3492.e4
Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice.
Zhang M, Wang Y, Geng J, Zhou S, Xiao B
Cell reports 2019 Feb 5;26(6):1419-1431.e4
Cell reports 2019 Feb 5;26(6):1419-1431.e4
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration.
Wiszniak S, Schwarz Q
BMC neuroscience 2019 Apr 29;20(1):21
BMC neuroscience 2019 Apr 29;20(1):21
Functional Local Proprioceptive Feedback Circuits Initiate and Maintain Locomotor Recovery after Spinal Cord Injury.
Takeoka A, Arber S
Cell reports 2019 Apr 2;27(1):71-85.e3
Cell reports 2019 Apr 2;27(1):71-85.e3
Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines.
Patil MJ, Hovhannisyan AH, Akopian AN
PloS one 2018;13(6):e0198601
PloS one 2018;13(6):e0198601
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells.
Alshawaf AJ, Viventi S, Qiu W, D'Abaco G, Nayagam B, Erlichster M, Chana G, Everall I, Ivanusic J, Skafidas E, Dottori M
Scientific reports 2018 Jan 12;8(1):603
Scientific reports 2018 Jan 12;8(1):603
Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo.
Kim S, Moon GJ, Oh YS, Park J, Shin WH, Jeong JY, Choi KS, Jin BK, Kholodilov N, Burke RE, Kim HJ, Ha CM, Lee SG, Kim SR
Experimental & molecular medicine 2018 Feb 9;50(2):e440
Experimental & molecular medicine 2018 Feb 9;50(2):e440
Genetic Tracing of Ca(v)3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System.
Bernal Sierra YA, Haseleu J, Kozlenkov A, Bégay V, Lewin GR
Frontiers in molecular neuroscience 2017;10:70
Frontiers in molecular neuroscience 2017;10:70
Treadmill exercise suppressed stress-induced dendritic spine elimination in mouse barrel cortex and improved working memory via BDNF/TrkB pathway.
Chen K, Zhang L, Tan M, Lai CS, Li A, Ren C, So KF
Translational psychiatry 2017 Mar 21;7(3):e1069
Translational psychiatry 2017 Mar 21;7(3):e1069
Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In Vivo.
Guimarães-Camboa N, Cattaneo P, Sun Y, Moore-Morris T, Gu Y, Dalton ND, Rockenstein E, Masliah E, Peterson KL, Stallcup WB, Chen J, Evans SM
Cell stem cell 2017 Mar 2;20(3):345-359.e5
Cell stem cell 2017 Mar 2;20(3):345-359.e5
Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway.
Price EA, Krasowska-Zoladek A, Nanda KK, Stachel SJ, Henze DA
Journal of neuroscience methods 2017 Apr 15;282:34-42
Journal of neuroscience methods 2017 Apr 15;282:34-42
KCNQ Potassium Channels Modulate Sensitivity of Skin Down-hair (D-hair) Mechanoreceptors.
Schütze S, Orozco IJ, Jentsch TJ
The Journal of biological chemistry 2016 Mar 11;291(11):5566-5575
The Journal of biological chemistry 2016 Mar 11;291(11):5566-5575
Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes.
Reed-Geaghan EG, Wright MC, See LA, Adelman PC, Lee KH, Koerber HR, Maricich SM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Apr 13;36(15):4362-76
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Apr 13;36(15):4362-76
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Usoskin D, Furlan A, Islam S, Abdo H, Lönnerberg P, Lou D, Hjerling-Leffler J, Haeggström J, Kharchenko O, Kharchenko PV, Linnarsson S, Ernfors P
Nature neuroscience 2015 Jan;18(1):145-53
Nature neuroscience 2015 Jan;18(1):145-53
Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
Gatto G, Dudanova I, Suetterlin P, Davies AM, Drescher U, Bixby JL, Klein R
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Mar 20;33(12):5399-410
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Mar 20;33(12):5399-410
Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
Vaegter CB, Jansen P, Fjorback AW, Glerup S, Skeldal S, Kjolby M, Richner M, Erdmann B, Nyengaard JR, Tessarollo L, Lewin GR, Willnow TE, Chao MV, Nykjaer A
Nature neuroscience 2011 Jan;14(1):54-61
Nature neuroscience 2011 Jan;14(1):54-61
Embryonic sympathoblasts transiently express TrkB in vivo and proliferate in response to brain-derived neurotrophic factor in vitro.
Straub JA, Sholler GL, Nishi R
BMC developmental biology 2007 Feb 19;7:10
BMC developmental biology 2007 Feb 19;7:10
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TrkB by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and mouse brain (cortex) tissue . PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TrkB by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkB at approximately 154 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat TrkB by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkB at approximately 95 kDa and 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TrkB in Mouse Spinal Cord. TrkB was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal processes and cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
