TA500386

antibody from OriGene

Targeting: NTRK2 TRKB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500386

Provider

OriGene

Proper citation

OriGene Cat#TA500386, RRID:AB_2155134

Product name

Anti-NTRK2 (TrkB ) mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)

Antibody type

Monoclonal

Description

Anti-NTRK2 (TrkB ) mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)

Host

Mouse

Conjugate

Unconjugated

Epitope

NTRK2

Isotype

IgG

Antibody clone number

OTI2E1

Vial size

100 µl

Concentration

1.17 mg/ml

References

Submitted references

Human brain proteins showing neuron-specific interactions with γ-secretase.

Inoue M, Hur JY, Kihara T, Teranishi Y, Yamamoto NG, Ishikawa T, Wiehager B, Winblad B, Tjernberg LO, Schedin-Weiss S
The FEBS journal 2015 Jul;282(14):2587-99

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTRK2 (RC205129, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK2.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-NTRK2 mouse monoclonal antibody (TA500386) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NTRK2(RC205129).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NTRK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500386)

Validation comment

IHC