13129-1-AP

antibody from Invitrogen Antibodies

Targeting: NTRK2 TRKB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13129-1-AP

Provider

Invitrogen Antibodies

Product name

TrkB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SRIWCSDPS PGIVAFPRLE PNSVDPENIT EIFIANQKRL EIINEDDVEA YVGLRNLTIV DSGLKFVAHK AFLKNSNLQH INFTRNKLTS LSRKHFRHLD LSELILVGNP FTCSCDIMWI KTLQEAKSSP DTQDLYCLNE SSKNIPLANL QIPNCGLPSA NLAAPNLTVE EGKSITLSCS VAGDPVPNMY WDVGNLVSKH MNETSHTQGS LRITNISSDD SGKQISCVAE NLVGEDQDSV NLTVHFAPTI TFLESPTSDH HWCIPFTVKG NPKPALQWFY NGAILNESKY ICTKIHVTNH TEYHGCLQLD NPTHMNNGDY TLIAKNEYGK DEKQISAHFM GWPGIDDGAN PNYPDVIYED YGTAANDIGD TTNRSNEIPS TDVTDKTGRE HLSV (41-433 aa encoded by BC031835)

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human stomach tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 13129-1-AP ( TrkB antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 13129-1-AP ( TrkB antibody at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TrkB (IP:13129-1-AP, 4ug; Detection:13129-1-AP 1:300) with mouse cerebellum tissue lysate 4000ug.