LS-C742085

antibody from LSBio

Targeting: MTR cblG

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C742085

Provider

LSBio

Product name

MS / MTR Antibody (clone 11G1D7) LS-C742085

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

11G1D7

Storage

Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

KO and Independent Western Blot validation of Human MTR Antibody (11G1D7) and Human MTR Antibody (3H1D9) with Human MTR recombinant protein. Lane 1: 25 ng Human MTR recombinant protein Lane 2: 5 ng Human MTR recombinant protein Lane 3: 1 ng Human MTR recombinant protein Lane 4: 25 ng Human MTR recombinant protein Lane 5: 5 ng Human MTR recombinant protein Lane 6: 1 ng Human MTR recombinant protein Primary Antibody: Lane 1~3: Human MTR Antibody (3H1D9) 1 µg/ml Lane 4~6: Human MTR Antibody (11G1D7) (H&L) [IRDye8°°] (Licor,926-32211)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

KO and Independent Western Blot validation of Human MTR Antibody (11G1D7) and Human MTR Antibody (3H1D9) with Hela cell lysates. Lane 1: 100 µg Wild-type Hela cell Lysate Lane 2: 100 µg MTR knockout Hela cell Lysate Lane 3: 100 µg Wild-type Hela cell Lysate Lane 4: 100 µg MTR knockout Hela cell Lysate Primary Antibody: Lane 1~2: Human MTR Antibody (3H1D9) 1 µg/ml Lane 3~4: Human MTR Antibody (11G1D7) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

KO and Independent Western Blot validation of Human MTR Antibody (11G1D7) and Human MTR Antibody (3H1D9) with Human MTR recombinant protein. Lane 1: 25 ng Human MTR recombinant protein Lane 2: 5 ng Human MTR recombinant protein Lane 3: 1 ng Human MTR recombinant protein Lane 4: 25 ng Human MTR recombinant protein Lane 5: 5 ng Human MTR recombinant protein Lane 6: 1 ng Human MTR recombinant protein Primary Antibody: Lane 1~3: Human MTR Antibody (3H1D9) 1 µg/ml Lane 4~6: Human MTR Antibody (11G1D7) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

KO and Independent Western Blot validation of Human MTR Antibody (11G1D7) and Human MTR Antibody (3H1D9) with Hela cell lysates. Lane 1: 100 µg Wild-type Hela cell Lysate Lane 2: 100 µg MTR knockout Hela cell Lysate Lane 3: 100 µg Wild-type Hela cell Lysate Lane 4: 100 µg MTR knockout Hela cell Lysate Primary Antibody: Lane 1~2: Human MTR Antibody (3H1D9) 1 µg/ml Lane 3~4: Human MTR Antibody (11G1D7) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

KO and Independent Western Blot validation of Human MTR Antibody (11G1D7) and Human MTR Antibody (3H1D9) with Hela cell lysates. Lane 1: 100 µg Wild-type Hela cell Lysate Lane 2: 100 µg MTR knockout Hela cell Lysate Lane 3: 100 µg Wild-type Hela cell Lysate Lane 4: 100 µg MTR knockout Hela cell Lysate Primary Antibody: Lane 1~2: Human MTR Antibody (3H1D9) 1 µg/ml Lane 3~4: Human MTR Antibody (11G1D7) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

KO and Independent Western Blot validation of Human MTR Antibody (11G1D7) and Human MTR Antibody (3H1D9) with Human MTR recombinant protein. Lane 1: 25 ng Human MTR recombinant protein Lane 2: 5 ng Human MTR recombinant protein Lane 3: 1 ng Human MTR recombinant protein Lane 4: 25 ng Human MTR recombinant protein Lane 5: 5 ng Human MTR recombinant protein Lane 6: 1 ng Human MTR recombinant protein Primary Antibody: Lane 1~3: Human MTR Antibody (3H1D9) 1 µg/ml Lane 4~6: Human MTR Antibody (11G1D7) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

KO and Independent Western Blot validation of Human MTR Antibody (11G1D7) and Human MTR Antibody (3H1D9) with Hela cell lysates. Lane 1: 100 µg Wild-type Hela cell Lysate Lane 2: 100 µg MTR knockout Hela cell Lysate Lane 3: 100 µg Wild-type Hela cell Lysate Lane 4: 100 µg MTR knockout Hela cell Lysate Primary Antibody: Lane 1~2: Human MTR Antibody (3H1D9) 1 µg/ml Lane 3~4: Human MTR Antibody (11G1D7) (H&L) [IRDye8°°] (Licor,926-32211)

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Standard curve of MTR Sandwich ELISA. The MTR Sandwich ELISA assay is developed by using Human MTR Antibody (11G1D7) and Biotin conjugated Human MTR Antibody (3H1D9) as capture and detect antibody, respectively. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA binding of Human MTR Antibody (11G1D7) with Human MTR recombinant protein. Coating antigen: MTR, 1 µg/ml. MTR antibody dilution start from 1000 ng/ml, EC50= 4.326 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Standard curve of MTR Sandwich ELISA. The MTR Sandwich ELISA assay is developed by using Human MTR Antibody (11G1D7) and Biotin conjugated Human MTR Antibody (3H1D9) as capture and detect antibody, respectively. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA binding of Human MTR Antibody (11G1D7) with Human MTR recombinant protein. Coating antigen: MTR, 1 µg/ml. MTR antibody dilution start from 1000 ng/ml, EC50= 4.326 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Standard curve of MTR Sandwich ELISA. The MTR Sandwich ELISA assay is developed by using Human MTR Antibody (11G1D7) and Biotin conjugated Human MTR Antibody (3H1D9) as capture and detect antibody, respectively. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA binding of Human MTR Antibody (11G1D7) with Human MTR recombinant protein. Coating antigen: MTR, 1 µg/ml. MTR antibody dilution start from 1000 ng/ml, EC50= 4.326 ng/ml.