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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- NB600-238 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-238, RRID:AB_2225871
- Product name
- Rabbit Polyclonal ELYS Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: ELYS Antibody [NB600-238] - Nuclear Extract (10 and 20 ug for WB; 1 mg for IP/WB) from HeLa cells. Antibodies: A) Affinity purified rabbit anti-ELYS antibody BL476 used at 0.5 ug/ml for WB and 4 ug/mg lysate for IP. B) Affinity purified rabbit anti-ELYS antibodies BL476 and BL475 were each used at 4 ug/mg extract for IP. Immunoprecipitated ELYS was detected by WB using BL475 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 3 to 5 minutes.
