GTX13141

antibody from GeneTex

Targeting: TNIK KIAA0551

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX13141

Provider

GeneTex

Proper citation

GeneTex Cat#GTX13141, RRID:AB_383061

Product name

TNIK antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

The Ste20 Family Kinases MAP4K4, MINK1, and TNIK Converge to Regulate Stress-Induced JNK Signaling in Neurons.

Larhammar M, Huntwork-Rodriguez S, Rudhard Y, Sengupta-Ghosh A, Lewcock JW
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Nov 15;37(46):11074-11084

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The essential role of TNIK gene amplification in gastric cancer growth.

Yu DH, Zhang X, Wang H, Zhang L, Chen H, Hu M, Dong Z, Zhu G, Qian Z, Fan J, Su X, Xu Y, Zheng L, Dong H, Yin X, Ji Q, Ji J
Oncogenesis 2014 Feb 24;2(2):e89

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Traf2- and Nck-interacting kinase is essential for Wnt signaling and colorectal cancer growth.

Shitashige M, Satow R, Jigami T, Aoki K, Honda K, Shibata T, Ono M, Hirohashi S, Yamada T
Cancer research 2010 Jun 15;70(12):5024-33

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

TNIK antibody validation by shRNA knock-downUpper panel: TNIK antibody (GTX13141)Lower panel: p84 antibody(GTX70220)A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of TNIK shRNA-transfected 293T cells5% SDS-PAGETNIK antibody (GTX13141) dilution: 1:5000p84 antibody(GTX70220) dilution: 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

41983

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TNIK antibody validation by shRNA knock-downUpper panel: TNIK antibody (GTX13141)Lower panel: p84 antibody(GTX70220)A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of TNIK shRNA-transfected 293T cells5% SDS-PAGETNIK antibody (GTX13141) dilution: 1:5000p84 antibody(GTX70220) dilution: 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TNIK antibody detects TNIK protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TNIK protein stained by TNIK antibody (GTX13141) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TNIK antibody detects TNIK protein at cytosol on mouse Colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse ColonTNIK antibody (GTX13141) dilution: 1:100

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TNIK antibody detects TNIK protein at cytosol on Mouse Kidney by immunohistochemical analysis. Sample: Paraffin-embedded Mouse Kidney TNIK antibody (GTX13141) dilution: 1:100

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TNIK antibody detects TNIK protein at cytosol on Mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded Mouse small intestine TNIK antibody (GTX13141) dilution: 1:100