CABT-B9334

antibody from Creative Diagnostics

Targeting: TAOK1 FLJ14314, KIAA1361, MAP3K16, MARKK, PSK2, TAO1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9334

Provider

Creative Diagnostics

Product name

Mouse anti-Rat TAO1 monoclonal antibody, clone 33/UBP2

Antibody type

Monoclonal

Description

Mitogen activated protein kinases (MAPKs) are critical components of several signal transduction pathways that convert extracellular stimuli into cellular responses. Four groups of MAPKs (ERKs, JNKs, p38, and ERK5) have been identified in mammalian cells. MAPK pathways contain a 3-kinase cascade consisting of a MAPK, a MAP/ERK kinase (MEK), and a MEK kinase (MEKK). MEKK phosphorylation of MEKs leads to activation and subsequent MEK-mediated phosphorylation of both Thr and Tyr residues at the Thr-X-Tyr dual phosphorylation motif of MAPKs. TAO1 was isolated from a rat cDNA library using the sequence from yeast Ste20p kinase. Sequence analysis shows that TAO1 contains an N-terminal Ser/Thr protein kinase domain, an acidic domain, and two serine-rich domains. The catalytic domain of TAO1 is 40% identical to the p21-activated kinases, PAK1 and PAK2, and 33% identical to MEKK1. TAO1 expression is highest in brain, but it is also detected in heart and lung. TAO1 can activate MEK3, MEK4, and MEK6 from the stress-responsive MAPK pathway, but not MEK1 or 2 from the classical MAPK pathway. Thus, TAO1 may be an important MEKK in the p38-containing stress-responsive MAPK pathway.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

33/UBP2

Vial size

50 _g

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.