Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C745490 - Provider product page
- Provider
- LSBio
- Product name
- MST1R / RON Antibody LS-C745490
- Antibody type
- Polyclonal
- Description
- Affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Anti-RON (RABBIT) Antibody. Lane 1: Protein Standard Opal Pre-stained Lane 2: Ron-GST. Lane 3: GST. Lane 4: A431 whole cell lysate Primary Antibody: Anti-RON (RABBIT) antibody at 1 ug/mL for overnight at 4°C. Secondary Antibody :Goat Anti-Rabbit Secondary HRP antibody at 1:70,000 for 30 min at RT. Block: MB-070 at RT for 30 min. Predicted/Observed size: ~152.3 kDa full length and 70 kDa cytoplasmic.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit anti-RON antibody. Lane 1: Phospho RON rProtein (cytoplasmic domain). Load: 0.05 µg per lane. Primary antibody: RON antibody at 1µg for overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit IgG secondary antibody at 1:40,000 for 45 min at RT. Block: MB-070 Fluorescent blocking buffer overnight at 4°C. Predicted/Observed size: 152 kDa (full legnth)/ ~70 kDa (cytoplasmic domain) RON. Other band(s): none.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Anti-RON (RABBIT) Antibody. Lane 1: Protein Standard Opal Pre-stained Lane 2: Ron-GST. Lane 3: GST. Lane 4: A431 whole cell lysate Primary Antibody: Anti-RON (RABBIT) antibody at 1 ug/mL for overnight at 4°C. Secondary Antibody :Goat Anti-Rabbit Secondary HRP antibody at 1:70,000 for 30 min at RT. Block: MB-070 at RT for 30 min. Predicted/Observed size: ~152.3 kDa full length and 70 kDa cytoplasmic.