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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX111958 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111958, RRID:AB_11162818
- Product name
- MELK antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: HeLa B: HCT116 7.5% SDS PAGE GTX111958 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MELK(GTX111958) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MELK antibody detects MELK protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MELK antibody (GTX111958) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MELK antibody detects MELK protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MELK antibody (GTX111958) diluted at 1:500.
