HPA017214

antibody from Atlas Antibodies

Targeting: MELK KIAA0175

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA017214

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA017214, RRID:AB_1853798

Product name

Anti-MELK

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLST
GAATPRTSQFTKYWTESNGVESKSLTPALCRTPAN
KLKNKENVYTPKSAVKNEEYFMFPEPKTPVNKNQH
KREILTTPNRYTTPSKARNQCLKETPIKIPVNSTG
TD


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and MELK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402376).

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.

Sample type

HUMAN