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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
- Other assay [2]
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- Product number
- TA801287 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA801287, RRID:AB_2625885
- Product name
- ALK mouse monoclonal antibody, clone OTI1A4 (discontinued and upgraded to UltraMAB UM800118)
- Antibody type
- Monoclonal
- Description
- ALK mouse monoclonal antibody, clone OTI1A4 (discontinued and upgraded to UltraMAB UM800118)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ALK
- Isotype
- IgG
- Antibody clone number
- OTI1A4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Inflammatory myofibroblastic tumour of the bladder in children: a review.
A novel, highly sensitive ALK antibody 1A4 facilitates effective screening for ALK rearrangements in lung adenocarcinomas by standard immunohistochemistry.
Extraordinary response to crizotinib in a woman with squamous cell lung cancer after two courses of failed chemotherapy.
Collin M, Charles A, Barker A, Khosa J, Samnakay N
Journal of pediatric urology 2015 Oct;11(5):239-45
Journal of pediatric urology 2015 Oct;11(5):239-45
A novel, highly sensitive ALK antibody 1A4 facilitates effective screening for ALK rearrangements in lung adenocarcinomas by standard immunohistochemistry.
Gruber K, Kohlhäufl M, Friedel G, Ott G, Kalla C
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2015 Apr;10(4):713-6
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2015 Apr;10(4):713-6
Extraordinary response to crizotinib in a woman with squamous cell lung cancer after two courses of failed chemotherapy.
Wang Q, He Y, Yang X, Wang Y, Xiao H
BMC pulmonary medicine 2014 May 15;14:83
BMC pulmonary medicine 2014 May 15;14:83
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from H2228 and A549 cell lines by using anti-ALK monoclonal antibody. (TA801287, 1:10,000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human non-small cell lung cancer sample with ALK translocation detected by FISH? using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human large B cell lymphoma with ALK translocation? using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded ALK-positive lung tumor xenograft? using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human non-small cell lung cancer sample with EML4-ALK translocation detected by PCR? using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of paraffin-embedded human cell line H1975 (upper left) and H2228 (upper right) on IHC antibody quality control slide using anti-ALK mouse monoclonal antibody TA801287 (1:400). The ALK rearrangement in H2228 cells is labeled with ALK Breakapart probe in FISH test (lower right, 60X) and the control of H1975 cell at the same FISH probe test (lower left, 60X).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemistry of ALK protein level by using anti-ALK antibody in human right cervical lymph node. Dilution: 1:200
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ALK-positive lung cancer tissue using anti-ALK mouse monoclonal antibody. (TA801287, 1:100 for 30 min at RT; heat-induced epitope retrieval by TEE, pH9.0)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ALK Luminex with 10D7 Capture (TA801306) and 1A4 Detection (TA801287) Antibodies. Substrate used: full length HEK293 cells expressed recombinant ALK protein (TP322485).
- Validation comment
- LMNX
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ALK Luminex with 15B2 Capture (TA801288) and 1A4 Detection (TA801287) Antibodies. Substrate used: full length HEK293 cells expressed recombinant ALK protein (TP322485).
- Validation comment
- LMNX
