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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00009943-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009943-M01, RRID:AB_425821
- Product name
- OXSR1 monoclonal antibody (M01), clone 2A2-1A2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant OXSR1.
- Antigen sequence
MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAY
CAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQC
HHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIK
HIVAKGEHKSGVLDESTIATILREVLEGLEYLHKN
GQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGG
DITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADI
WSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPP
SLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRP
TAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERA
KKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEG
KAAISQLRSPRVKESISNSELFPTTDPVGTLLQVP
EQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQA
LSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFT
PGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIV
EEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLS
IS- Isotype
- IgG
- Antibody clone number
- 2A2-1A2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Generation and analysis of knock-in mice carrying pseudohypoaldosteronism type II-causing mutations in the cullin 3 gene.
ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney.
Araki Y, Rai T, Sohara E, Mori T, Inoue Y, Isobe K, Kikuchi E, Ohta A, Sasaki S, Uchida S
Biology open 2015 Oct 21;4(11):1509-17
Biology open 2015 Oct 21;4(11):1509-17
ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney.
Naguro I, Umeda T, Kobayashi Y, Maruyama J, Hattori K, Shimizu Y, Kataoka K, Kim-Mitsuyama S, Uchida S, Vandewalle A, Noguchi T, Nishitoh H, Matsuzawa A, Takeda K, Ichijo H
Nature communications 2012;3:1285
Nature communications 2012;3:1285
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- OXSR1 monoclonal antibody (M01), clone 2A2-1A2 Western Blot analysis of OXSR1 expression in A-431 ( Cat # L015V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged OXSR1 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to OXSR1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
