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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00055170-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055170-M02, RRID:AB_10965273
- Product name
- PRMT6 monoclonal antibody (M02), clone 3C3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant PRMT6.
- Antigen sequence
MIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTG
ILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGL
EDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHE
SMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQ
MLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHS
EIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEA
GVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKP
LVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSG
EITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAME
D- Isotype
- IgG
- Antibody clone number
- 3C3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PRMT6 monoclonal antibody (M02), clone 3C3. Western Blot analysis of PRMT6 expression in MCF-7.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PRMT6 expression in transfected 293T cell line by PRMT6 monoclonal antibody (M02), clone 3C3.Lane 1: PRMT6 transfected lysate (Predicted MW: 35.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PRMT6 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PRMT6 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
