AF432

antibody from Novus Biologicals

Targeting: EPHB3 ETK2, Hek2, Tyro6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF432

Provider

Novus Biologicals

Product name

Goat Polyclonal EphB3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human EphB3 is observed and less than 1% cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphA4, rmEphB2, rmEphB4, and rmEphB6 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing.

Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, Moonah S
Cellular and molecular gastroenterology and hepatology 2020;10(1):101-112

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Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.

O'Neill AK, Kindberg AA, Niethamer TK, Larson AR, Ho HH, Greenberg ME, Bush JO
The Journal of cell biology 2016 Oct 24;215(2):217-229

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Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.

Feng Y, Sakamoto N, Wu R, Liu JY, Wiese A, Green ME, Green M, Akyol A, Roy BC, Zhai Y, Cho KR, Fearon ER
PLoS genetics 2015 Nov;11(11):e1005638

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Distinct fibroblast lineages determine dermal architecture in skin development and repair.

Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons BD, Charalambous M, Ferron SR, Herault Y, Pavlovic G, Ferguson-Smith AC, Watt FM
Nature 2013 Dec 12;504(7479):277-281

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Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.

Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
Biochemical and biophysical research communications 2011 Feb 25;405(4):521-6

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Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism.

Bush JO, Soriano P
Genes & development 2009 Jul 1;23(13):1586-99

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The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.

Yang NY, Pasquale EB, Owen LB, Ethell IM
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86

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EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.

Holmberg J, Genander M, Halford MM, Annerén C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisén J
Cell 2006 Jun 16;125(6):1151-63

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EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.

Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
Journal of cell science 2006 Feb 1;119(Pt 3):559-70

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse EphB3 by Western Blot. Detection of Mouse EphB3 by Western Blot.