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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB432 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse EphB3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) EphB3 is observed and no cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphB4, rmEphB2, rmEphB7, rmEphA4, rmEphA3, rhEphB2, or rhEphA5 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P54754- Isotype
- IgG
- Antibody clone number
- 521002
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation.
Mimche PN, Brady LM, Keeton S, Fenne DS, King TP, Quicke KM, Hudson LE, Lamb TJ
PloS one 2015;10(9):e0138835
PloS one 2015;10(9):e0138835
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse EphB3 by Western Blot. Western blot shows lysates of mouse embryo (E19) tissue under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Rat Anti-Mouse EphB3 Monoclonal Antibody (Catalog # MAB432) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for EphB3 was detected at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
