GTX114462

antibody from GeneTex

Targeting: H1-0 H1.0, H1F0, H1FV

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX114462

Provider

GeneTex

Proper citation

GeneTex Cat#GTX114462, RRID:AB_11173658

Product name

Histone H1.0 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Temporally and Spatially Regulated Expression of the Linker Histone H1fx During Mouse Development.

Ichihara-Tanaka K, Kadomatsu K, Kishida S
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Sep;65(9):513-530

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Nucleosome-nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity.

Shimamoto Y, Tamura S, Masumoto H, Maeshima K
Molecular biology of the cell 2017 Jun 1;28(11):1580-1589

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Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.

Kuo HF, Liu PL, Chong IW, Liu YP, Chen YH, Ku PM, Li CY, Chen HH, Chiang HC, Wang CL, Chen HJ, Chen YC, Hsieh CC
PloS one 2016;11(5):e0156059

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Celastrol prevents circulatory failure via induction of heme oxygenase-1 and heat shock protein 70 in endotoxemic rats.

Wang YL, Lam KK, Cheng PY, Lee YM
Journal of ethnopharmacology 2015 Mar 13;162:168-75

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 12% SDS PAGE GTX114462 diluted at 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H1 antibody detects Histone H1 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H1 antibody detects Histone H1 protein by western blot analysis. HepG2 whole cell extracts and nuclear extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) at a dilution of 1:10000 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H1.0 antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H1.0 antibody (GTX114462) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H1.0 antibody (GTX114462) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H1.0 antibody (GTX114462) diluted at 1:500.