Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1199 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TWEAK R/TNFRSF12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse TWEAK R/TNFRSF12 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9NP84- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TWEAK/Fn14 system and crescent formation in IgA nephropathy.
Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
Sasaki Y, Shimizu Y, Suzuki Y, Horikoshi S, Tomino Y
BMC nephrology 2015 Mar 14;16:27
BMC nephrology 2015 Mar 14;16:27
Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
Gaudineau B, Fougère M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
Journal of cell science 2012 Oct 1;125(Pt 19):4475-86
Journal of cell science 2012 Oct 1;125(Pt 19):4475-86
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-depend-ent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Anti-body. Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199-TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND50 is typically 0.3-1.2 µg/mL.
