PA5-52473

antibody from Invitrogen Antibodies

Targeting: CCAR1 CARP-1, CARP1, FLJ10590

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52473

Provider

Invitrogen Antibodies

Product name

CCAR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KDVEENVGLI VYNGAMVDVG SLLQKLEKSE KVRAEVEQKL QLLEEKTDED EKTILNLENS NKSLSGELRE VKKDLSQLQE NLKISENMNL QFENQMNKTI RNLSTVMDEI HTVLKKDNVK NED

Concentration

0.1 mg/mL

References

Submitted references

Circular RNA circRIMS1 Acts as a Sponge of miR-433-3p to Promote Bladder Cancer Progression by Regulating CCAR1 Expression.

Wang F, Fan M, Cai Y, Zhou X, Tai S, Yu Y, Wu H, Zhang Y, Liu J, Huang S, He N, Hu Z, Jin X
Molecular therapy. Nucleic acids 2020 Dec 4;22:815-831

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CCAR1 in human cell line U-251 MG using a CCAR1 Polyclonal Antibody (Product # PA5-52473).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CCAR1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a CCAR1 Polyclonal Antibody (Product # PA5-52473).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CCAR1 in human lymph node using a CCAR1 Polyclonal Antibody (Product # PA5-52473) shows strong nuclear positivity in germinal center and non-germinal center cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 CCAR1 Is Directly Targeted by miR-433-3p and Serves as a Tumor Promoter in Bladder Cancer (A) Significantly differentially expressed mRNAs are presented as a clustered heatmap in T24 cells (negative control versus sh-circRIMS1). Red dots represent upregulated genes, and green dots indicate downregulated genes in the volcano plot. (B) Overlap of downregulated mRNAs identified by RNA sequencing and target genes of miR-433-3p predicted from TargetScan and StarBase databases. (C) Expression levels of CCAR1, CEP135, and NEGR1 in control or circRIMS1-knockdown cells were further verified. (D) The biological effect of CCAR1 was determined by siRNA transfections and Annexin V-FITC/PI staining. (E) Luciferase reporter assay confirmed the interaction between miR-433-3p and the CCAR1 3' UTR. miR-433-3p mimics (or negative control mimic) were cotransfected with wild-type or mutated CCAR1 3' UTR luciferase reporter vector into HEK293 cells. (F and G) Cell viability and colony-forming ability in control or CCAR1-deficient cells evaluated by CCK-8 assay (F) and colony-formation assay (G), respectively. (H and I) To confirm the role of CCAR1 in cell migration and invasion abilities, Transwell migration and invasion assays (H) and wound-healing assay (I) were used to detect cell migration ability. (J) mRNA expression levels of two Wnt signaling target genes (c-Myc and survivin) were downregulated by CCAR1 depletion, as determined by qRT-PCR. (K) Expression of CCAR1, c-Myc, survivin,