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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-52473 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCAR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KDVEENVGLI VYNGAMVDVG SLLQKLEKSE KVRAEVEQKL QLLEEKTDED EKTILNLENS NKSLSGELRE VKKDLSQLQE NLKISENMNL QFENQMNKTI RNLSTVMDEI HTVLKKDNVK NED Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 94%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Circular RNA circRIMS1 Acts as a Sponge of miR-433-3p to Promote Bladder Cancer Progression by Regulating CCAR1 Expression.
Wang F, Fan M, Cai Y, Zhou X, Tai S, Yu Y, Wu H, Zhang Y, Liu J, Huang S, He N, Hu Z, Jin X
Molecular therapy. Nucleic acids 2020 Dec 4;22:815-831
Molecular therapy. Nucleic acids 2020 Dec 4;22:815-831
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCAR1 in human cell line U-251 MG using a CCAR1 Polyclonal Antibody (Product # PA5-52473).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCAR1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a CCAR1 Polyclonal Antibody (Product # PA5-52473).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCAR1 in human lymph node using a CCAR1 Polyclonal Antibody (Product # PA5-52473) shows strong nuclear positivity in germinal center and non-germinal center cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 CCAR1 Is Directly Targeted by miR-433-3p and Serves as a Tumor Promoter in Bladder Cancer (A) Significantly differentially expressed mRNAs are presented as a clustered heatmap in T24 cells (negative control versus sh-circRIMS1). Red dots represent upregulated genes, and green dots indicate downregulated genes in the volcano plot. (B) Overlap of downregulated mRNAs identified by RNA sequencing and target genes of miR-433-3p predicted from TargetScan and StarBase databases. (C) Expression levels of CCAR1, CEP135, and NEGR1 in control or circRIMS1-knockdown cells were further verified. (D) The biological effect of CCAR1 was determined by siRNA transfections and Annexin V-FITC/PI staining. (E) Luciferase reporter assay confirmed the interaction between miR-433-3p and the CCAR1 3' UTR. miR-433-3p mimics (or negative control mimic) were cotransfected with wild-type or mutated CCAR1 3' UTR luciferase reporter vector into HEK293 cells. (F and G) Cell viability and colony-forming ability in control or CCAR1-deficient cells evaluated by CCK-8 assay (F) and colony-formation assay (G), respectively. (H and I) To confirm the role of CCAR1 in cell migration and invasion abilities, Transwell migration and invasion assays (H) and wound-healing assay (I) were used to detect cell migration ability. (J) mRNA expression levels of two Wnt signaling target genes (c-Myc and survivin) were downregulated by CCAR1 depletion, as determined by qRT-PCR. (K) Expression of CCAR1, c-Myc, survivin,
