13271-1-AP

antibody from Invitrogen Antibodies

Targeting: NGEF ARHGEF27

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13271-1-AP

Provider

Invitrogen Antibodies

Product name

NGEF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: METRESEDL EKTRRKSASD QWNTDNEPAK VKPELLPEKE ETSQADQDIQ DKEPHCHIPI KRNSIFNRSI RRKSKAKARD NPERNASCLA DSQDNGKSVN EPLTLNIPWS RTPPCRTAMQ TDPGAQEMSE SSSTPGNGAT PEEWPALADS PTTLTEALRM IHPIPADSWR NLIEQIGLLY QEYRDKSTLQ EIETRRQQDA EIEDNTNGSP ASEDTPEEEE EEEEEEEPAS PPERKTLPQI CLLSNPHSRF NLWQDLPEIR SSGVLEILQP EEIK (1-273 aa encoded by BC031573)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 13271-1-AP ( NGEF antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 13271-1-AP ( NGEF antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NGEF (IP:13271-1-AP, 4ug; Detection:13271-1-AP 1:500) with mouse brain tissue lysate 4000ug.