MA5-29406

antibody from Invitrogen Antibodies

Targeting: LXN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29406

Provider

Invitrogen Antibodies

Product name

Latexin Recombinant Rabbit Monoclonal Antibody (101)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

101

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Latexin in Lane A: A549 Whole Cell Lysate (30 µg). Samples were probed using a Latexin Monoclonal Antibody (Product # MA5-29406) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:26 kDa. Observed band size:26 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Latexin Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29406) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human Latexin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Latexin Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29406, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human Latexin expression on MCF-7 cells. The cells were treated according to manufacturer’s manual, stained with Latexin Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29406), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Latexin Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate 2 µL with Latexin Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29406) and 15 µL of 50 % Protein G agarose. Primary antibody: Latexin Recombinant Rabbit Monoclonal Antibody (101), at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25.7 kDa. Observed band size: 25.7 kDa.