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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52994PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52994PU-N, RRID:AB_11143687
- Product name
- anti Mimecan (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 246-276 amino acids from the C-terminal region of Human Mimecan.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 0.26 mg/mL
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of OGN Antibody (C-term) Cat.-No AP52994PU-N in CEM cell line lysates (35ug/lane). This demonstrates the OGN antibody detected the OGN protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of OGN (arrow) using rabbit polyclonal OGN Antibody (C-term) Cat.-No AP52994PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OGN gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue reacted with OGN Antibody (C-term) Cat.-No AP52994PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.T
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells using OGN Antibody (C-term) Cat.-No AP52994PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
