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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [3]
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Validation data
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- Product number
- TA502768 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502768, RRID:AB_11128375
- Product name
- NFKBIZ mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)
- Antibody type
- Monoclonal
- Description
- NFKBIZ mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NFKBIZ
- Isotype
- IgG
- Antibody clone number
- OTI1E8
- Vial size
- 100 µl
- Concentration
- 0.58 mg/ml
Submitted references A mix of S and ΔS variants of STAT3 enable survival of activated B-cell-like diffuse large B-cell lymphoma cells in culture.
Zheng M, Turton KB, Zhu F, Li Y, Grindle KM, Annis DS, Lu L, Drennan AC, Tweardy DJ, Bharadwaj U, Mosher DF, Rui L
Oncogenesis 2016 Jan 4;4:e184
Oncogenesis 2016 Jan 4;4:e184
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFKBIZ (RC219121, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIZ.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-NFKBIZ antibody(TA502768),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-NFKBIZ antibody(TA502768),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC219121 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NFKBIZ antibody(TA502768), and then analyzed by flow cytometry.
- Validation comment
- FC
