Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- AF5049 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal 5T4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse 5T4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human 5T4 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references WAIF1 Is a Cell-Surface CTHRC1 Binding Protein Coupling Bone Resorption and Formation.
The Stem Cell Marker Lgr5 Defines a Subset of Postmitotic Neurons in the Olfactory Bulb.
A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors.
Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
Matsuoka K, Kohara Y, Naoe Y, Watanabe A, Ito M, Ikeda K, Takeshita S
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Aug;33(8):1500-1512
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Aug;33(8):1500-1512
The Stem Cell Marker Lgr5 Defines a Subset of Postmitotic Neurons in the Olfactory Bulb.
Yu Y, Moberly AH, Bhattarai JP, Duan C, Zheng Q, Li F, Huang H, Olson W, Luo W, Wen T, Yu H, Ma M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9403-9414
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9403-9414
A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors.
Takahashi H, Ogawa Y, Yoshihara S, Asahina R, Kinoshita M, Kitano T, Kitsuki M, Tatsumi K, Okuda M, Tatsumi K, Wanaka A, Hirai H, Stern PL, Tsuboi A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 3;36(31):8210-27
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 3;36(31):8210-27
Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
Rosu-Myles M, She YM, Fair J, Muradia G, Mehic J, Menendez P, Prasad SS, Cyr TD
PloS one 2012;7(6):e38954
PloS one 2012;7(6):e38954
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of 5T4 in D3 Mouse Cell Line by Flow Cytometry. Retinoic acid-treated D3 mouse embryonic stem cell line was stained with Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).