10200-1-AP

antibody from Proteintech Group

Targeting: WTAP KIAA0105, MGC3925, Mum2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

10200-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10200-1-AP, RRID:AB_2216349

Product name

WTAP antibody

Antibody type

Polyclonal

Description

KD/KO validated WTAP antibody (Cat. #10200-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Downregulation of Wtap causes dilated cardiomyopathy and heart failure.

Shi L, Li X, Zhang M, Qin C, Zhang Z, Chen Z
Journal of molecular and cellular cardiology 2024 Mar;188:38-51

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Dynamics of N6-methyladenosine modification during Alzheimer's disease development.

Gao S, Wang Y, Li X, Liang Y, Jin Z, Yang B, Yuan TF, Tian H, Peng B, Rao Y
Heliyon 2024 Mar 30;10(6):e26911

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BIRC5 facilitates cisplatin-chemoresistance in a m(6)A-dependent manner in ovarian cancer.

Fan Y, Pan Y, Jia L, Gu S, Liu B, Mei Z, Lv C, Huang H, Zhu G, Deng Q
Cancer medicine 2024 Jan;13(1):e6811

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Circular RNA-circPan3 attenuates cardiac hypertrophy via miR-320-3p/HSP20 axis.

Fang X, Ao X, Xiao D, Wang Y, Jia Y, Wang P, Li M, Wang J
Cellular & molecular biology letters 2024 Jan 3;29(1):3

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Exosome-based WTAP siRNA delivery ameliorates myocardial ischemia-reperfusion injury.

Yin T, Wang N, Jia F, Wu Y, Gao L, Zhang J, Hou R
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2024 Apr;197:114218

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Targeting FTO induces colorectal cancer ferroptotic cell death by decreasing SLC7A11/GPX4 expression.

Qiao Y, Su M, Zhao H, Liu H, Wang C, Dai X, Liu L, Liu G, Sun H, Sun M, Wang J, Li Z, Fan J, Zhang Q, Li C, Situ F, Xue J, Jia Z, Zhang C, Zhang S, Shan C
Journal of experimental & clinical cancer research : CR 2024 Apr 10;43(1):108

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WTAP affects intracranial aneurysm progression by regulating m6A methylation modification.

Yuan X, Bao Q, Lu B, Xiang C, Hou X, Wei W
Open medicine (Warsaw, Poland) 2023;18(1):20230818

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ZBTB7C m6A modification incurred by METTL3 aberration promotes osteosarcoma progression.

An X, Wu W, Yang L, Dong J, Liu B, Guo J, Chen J, Guo B, Cao W, Jiang Q
Translational research : the journal of laboratory and clinical medicine 2023 Sep;259:62-71

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HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5.

Huo FC, Zhu ZM, Du WQ, Pan YJ, Jiang X, Kang MJ, Liu BW, Mou J, Pei DS
Pharmacological research 2023 Sep;195:106863

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Multi-Omics Analysis Reveals Translational Landscapes and Regulations in Mouse and Human Oocyte Aging.

Huang J, Chen P, Jia L, Li T, Yang X, Liang Q, Zeng Y, Liu J, Wu T, Hu W, Kee K, Zeng H, Liang X, Zhou C
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Sep;10(26):e2301538

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Stage-specific requirement for m(6)A RNA methylation during cardiac differentiation of pluripotent stem cells.

Dong S, Sun Y, Liu C, Li Y, Yu S, Zhang Q, Xu Y
Differentiation; research in biological diversity 2023 Sep-Oct;133:77-87

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Dynamic N6-methyladenosine RNA methylation landscapes reveal epi-transcriptomic modulation induced by ammonia nitrogen exposure in the Pacific whiteleg shrimp Litopenaeus vannamei.

Lei Y, Yuan Z, Zeng Q, Wan B, Liu J, Wang W
Journal of hazardous materials 2023 Sep 15;458:131996

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WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation.

Song K, Sun H, Tu B, Zhou Y, Lin LC, Liu ZY, Li R, Yang JJ, Zhang Y, Zhao JY, Tao H
iScience 2023 Oct 20;26(10):107931

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Comprehensive Transcriptomic Profiling of m6A Modification in Age-Related Hearing Loss.

Feng M, Zhou X, Hu Y, Zhang J, Yang T, Chen Z, Yuan W
Biomolecules 2023 Oct 18;13(10)

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Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2-ERK depending on downregulation of proteasome-related genes.

Li X, Liu C, Zhang Z, Li X, Yao Z, Dong Y, Wang X, Chen Z
The Journal of biological chemistry 2023 Nov;299(11):105301

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m(6) A promotes planarian regeneration.

Cui G, Zhou JY, Ge XY, Sun BF, Song GG, Wang X, Wang XZ, Zhang R, Wang HL, Jing Q, Koziol MJ, Zhao YL, Zeng A, Zhang WQ, Han DL, Yang YG, Yang Y
Cell proliferation 2023 May;56(5):e13481

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Overexpression of VIRMA confers vulnerability to breast cancers via the m(6)A-dependent regulation of unfolded protein response.

Lee Q, Song R, Phan DAV, Pinello N, Tieng J, Su A, Halstead JM, Wong ACH, van Geldermalsen M, Lee BS, Rong B, Cook KM, Larance M, Liu R, Lan F, Tiffen JC, Wong JJ
Cellular and molecular life sciences : CMLS 2023 May 19;80(6):157

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Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m(6)A-dependent recognition of Nrf2 mRNA mediated by SND1.

Zheng J, Zhang Q, Zhao Z, Qiu Y, Zhou Y, Wu Z, Jiang C, Wang X, Jiang X
Journal of experimental & clinical cancer research : CR 2023 May 19;42(1):127

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Deficiency of WTAP in islet beta cells results in beta cell failure and diabetes in mice.

Li X, Yang Y, Li Z, Wang Y, Qiao J, Chen Z
Diabetologia 2023 Jun;66(6):1084-1096

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FTO-dependent m(6)A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke.

Li B, Xi W, Bai Y, Liu X, Zhang Y, Li L, Bian L, Liu C, Tang Y, Shen L, Yang L, Gu X, Xie J, Zhou Z, Wang Y, Yu X, Wang J, Chao J, Han B, Yao H
Nature communications 2023 Jan 30;14(1):489

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[m(6)A methylase WTAP participates in renal ischemia-reperfusion injury by regulating FOXO1 expression].

Zhao G, Li H, Zhang H, Xiao K, Yang H, Li Z, Fu C
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2023 Dec 20;43(12):2035-2042

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Coilin mediates m6A RNA methylation through phosphorylation of METTL3.

McLaurin DM, Tucker SK, Hebert MD
Biology open 2023 Dec 15;12(12)

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Profile of N6-methyladenosine of Pb-exposed neurons presents epitranscriptomic alterations in PI3K-AKT pathway-associated genes.

Ai S, Li D, Gu X, Xu Y, Wang Y, Wang HL, Chen XT
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Aug;178:113821

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Characterization of m(6)A modifiers and RNA modifications in uterine fibroids.

George JW, Cancino RA, Miller JLG, Qiu F, Lin Q, Rowley MJ, Chennathukuzhi VM, Davis JS
bioRxiv : the preprint server for biology 2023 Aug 9;

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A lncRNA from the FTO locus acts as a suppressor of the m(6)A writer complex and p53 tumor suppression signaling.

Zhang J, Wei J, Sun R, Sheng H, Yin K, Pan Y, Jimenez R, Chen S, Cui XL, Zou Z, Yue Z, Emch MJ, Hawse JR, Wang L, He HH, Xia S, Han B, He C, Huang H
Molecular cell 2023 Aug 3;83(15):2692-2708.e7

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A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression.

Yan C, Xiong J, Zhou Z, Li Q, Gao C, Zhang M, Yu L, Li J, Hu MM, Zhang CS, Cai C, Zhang H, Zhang J
eLife 2023 Aug 17;12

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METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions.

Li Y, He X, Lu X, Gong Z, Li Q, Zhang L, Yang R, Wu C, Huang J, Ding J, He Y, Liu W, Chen C, Cao B, Zhou D, Shi Y, Chen J, Wang C, Zhang S, Zhang J, Ye J, You H
Nature communications 2022 Oct 26;13(1):6350

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m(6)A modification confers thermal vulnerability to HPV E7 oncotranscripts via reverse regulation of its reader protein IGF2BP1 upon heat stress.

Wang L, Zhan G, Maimaitiyiming Y, Su Y, Lin S, Liu J, Su K, Lin J, Shen S, He W, Wang F, Chen J, Sun S, Xue Y, Gu J, Chen X, Zhang J, Zhang L, Wang Q, Chang KJ, Chiou SH, Björklund M, Naranmandura H, Cheng X, Hsu CH
Cell reports 2022 Oct 25;41(4):111546

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A single N6-methyladenosine site regulates lncRNA HOTAIR function in breast cancer cells.

Porman AM, Roberts JT, Duncan ED, Chrupcala ML, Levine AA, Kennedy MA, Williams MM, Richer JK, Johnson AM
PLoS biology 2022 Nov;20(11):e3001885

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Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma.

Kong F, Wang K, Wang L
Discover oncology 2022 Nov 25;13(1):131

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WTAP Targets the METTL3 m(6)A-Methyltransferase Complex to Cytoplasmic Hepatitis C Virus RNA to Regulate Infection.

Sacco MT, Bland KM, Horner SM
Journal of virology 2022 Nov 23;96(22):e0099722

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Dynamic control of chromatin-associated m(6)A methylation regulates nascent RNA synthesis.

Xu W, He C, Kaye EG, Li J, Mu M, Nelson GM, Dong L, Wang J, Wu F, Shi YG, Adelman K, Lan F, Shi Y, Shen H
Molecular cell 2022 Mar 17;82(6):1156-1168.e7

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Expression pattern of alkB homolog 5 in goat testis and its role in spermatogonial stem cells.

Liu Z, Cai Y, Deng M, Li D, Leng Q, Shi L, Tang Y, Wang F, Wan Y
Cell and tissue research 2022 Jan;387(1):131-142

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YTHDC1 regulates distinct post-integration steps of HIV-1 replication and is important for viral infectivity.

N'Da Konan S, Ségéral E, Bejjani F, Bendoumou M, Ait Said M, Gallois-Montbrun S, Emiliani S
Retrovirology 2022 Jan 31;19(1):4

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The expression of m6A enzymes in the hippocampus of diabetic cognitive impairment mice and the possible improvement of YTHDF1.

Li M, Zhong X, Zhao Z, Zeng Z, Yuan Q, Xiao X, Zu X, Liu J
Brain research 2022 Feb 15;1777:147766

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Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH).

Li X, Ding K, Li X, Yuan B, Wang Y, Yao Z, Wang S, Huang H, Xu B, Xie L, Deng T, Chen XW, Chen Z
Nature communications 2022 Aug 4;13(1):4549

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PGE(2) -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.

Tao X, Du R, Guo S, Feng X, Yu T, OuYang Q, Chen Q, Fan X, Wang X, Guo C, Li X, Xue F, Chen S, Tong M, Lazarus M, Zuo S, Yu Y, Shen Y
The EMBO journal 2022 Aug 16;41(16):e110439

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METTL3-mediated m(6)A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

Jiang L, Liu X, Hu X, Gao L, Zeng H, Wang X, Huang Y, Zhu W, Wang J, Wen J, Meng X, Wu Y
Molecular therapy : the journal of the American Society of Gene Therapy 2022 Apr 6;30(4):1721-1740

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Vitamin B(12) Deficiency Dysregulates m6A mRNA Methylation of Genes Involved in Neurological Functions.

Mosca P, Robert A, Alberto JM, Meyer M, Kundu U, Hergalant S, Umoret R, Coelho D, Guéant JL, Leheup B, Dreumont N
Molecular nutrition & food research 2021 Sep;65(17):e2100206

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Degradation of WTAP blocks antiviral responses by reducing the m(6) A levels of IRF3 and IFNAR1 mRNA.

Ge Y, Ling T, Wang Y, Jia X, Xie X, Chen R, Chen S, Yuan S, Xu A
EMBO reports 2021 Nov 4;22(11):e52101

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METTL3 regulates heterochromatin in mouse embryonic stem cells.

Xu W, Li J, He C, Wen J, Ma H, Rong B, Diao J, Wang L, Wang J, Wu F, Tan L, Shi YG, Shi Y, Shen H
Nature 2021 Mar;591(7849):317-321

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m(6)A RNA methylation regulates the fate of endogenous retroviruses.

Chelmicki T, Roger E, Teissandier A, Dura M, Bonneville L, Rucli S, Dossin F, Fouassier C, Lameiras S, Bourc'his D
Nature 2021 Mar;591(7849):312-316

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EGR2-mediated regulation of m(6)A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization.

Ying Y, Ma X, Fang J, Chen S, Wang W, Li J, Xie H, Wu J, Xie B, Liu B, Wang X, Zheng X, Xie L
Cell death & disease 2021 Jul 29;12(8):750

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Integrated analysis of m(6)A mRNA methylation in rats with monocrotaline-induced pulmonary arterial hypertension.

Zeng Y, Huang T, Zuo W, Wang D, Xie Y, Wang X, Xiao Z, Chen Z, Liu Q, Liu N, Xiao Y
Aging 2021 Jul 26;13(14):18238-18256

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Reprogramming of m(6)A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia.

Wang YJ, Yang B, Lai Q, Shi JF, Peng JY, Zhang Y, Hu KS, Li YQ, Peng JW, Yang ZZ, Li YT, Pan Y, Koeffler HP, Liao JY, Yin D
RNA biology 2021 Jan;18(1):131-143

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The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N(6)-methyladenosine methylation of Parp10 mRNA.

Gao XQ, Zhang YH, Liu F, Ponnusamy M, Zhao XM, Zhou LY, Zhai M, Liu CY, Li XM, Wang M, Shan C, Shan PP, Wang Y, Dong YH, Qian LL, Yu T, Ju J, Wang T, Wang K, Chen XZ, Wang YH, Zhang J, Li PF, Wang K
Nature cell biology 2020 Nov;22(11):1319-1331

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WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1.

Chen Y, Peng C, Chen J, Chen D, Yang B, He B, Hu W, Zhang Y, Liu H, Dai L, Xie H, Zhou L, Wu J, Zheng S
Molecular cancer 2019 Aug 22;18(1):127

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Zc3h13 Regulates Nuclear RNA m(6)A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Wen J, Lv R, Ma H, Shen H, He C, Wang J, Jiao F, Liu H, Yang P, Tan L, Lan F, Shi YG, He C, Shi Y, Diao J
Molecular cell 2018 Mar 15;69(6):1028-1038.e6

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A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Moindrot B, Cerase A, Coker H, Masui O, Grijzenhout A, Pintacuda G, Schermelleh L, Nesterova TB, Brockdorff N
Cell reports 2015 Jul 28;12(4):562-72

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 10200-1-AP(WTAP antibody) at dilution of 1:1000

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The WTAP antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human WTAP. This antibody recognizes human,mouse,rat antigen. The WTAP antibody has been validated for the following applications: ELISA, IP, WB analysis.

Appendix

10200-1-AP.pdf

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