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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6583 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6583, RRID:AB_1580583
- Product name
- SH3BP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of SH3BP1.
- Antigen sequence
C-PQPRPRSLASETN- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The DNA sequence of human chromosome 22.
Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP
Nature 1999 Dec 2;402(6761):489-95
Nature 1999 Dec 2;402(6761):489-95
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SH3BP1 polyclonal antibody (Cat # PAB6583) staining (1 ug/mL) of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SH3BP1 polyclonal antibody (Cat # PAB6583) (0.3 ug/mL) staining of paraffin embedded human kidney. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
