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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21851 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH3BP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A549, HeLa, Jurkat, NT2D1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SH3BP1 Polyclonal Antibody (Product # PA5-21851). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: JurKat . 7.5% SDS PAGE. SH3BP1 Polyclonal Antibody (Product # PA5-21851) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SH3 domain-binding protein 1 in methanol-fixed HeLa cells using a SH3 domain-binding protein 1 polyclonal antibody (Product # PA5-21851) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using SH3BP1 antibody (Product # PA5-21851) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH3BP1 Polyclonal Antibody detects SH3BP1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. SH3BP1 Polyclonal Antibody (Product # PA5-21851) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
