A700-068

antibody from Invitrogen Antibodies

Targeting: TNFRSF18 AITR, CD357, GITR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-068

Provider

Invitrogen Antibodies

Product name

GITR/TNFRSF18 Recombinant Rabbit Monoclonal Antibody (BLR068G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR068G

Vial size

100 µL

Concentration

1000 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 µg) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody [BLR057F] (Product # A700-057).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GITR/TNFRSF18 by immunocytochemistry.Sample: FFPE section of RPMI-8226 cells.Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used at 1:200. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4). Counterstain: DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GITR/TNFRSF18 by immunohistochemistry. AntibodySample: FFPE section of tonsil.Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (BLR068G) (A700-068 lot 1) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GITR/TNFRSF18 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal [BLR068G] (Product #A700-068; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GITR/TNFRSF18 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used for IP at 6 µL per reaction. GITR/TNFRSF18 was also immunoprecipitated by rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BL-1066-1D11]. For blotting immunoprecipitated GITR/TNFRSF18 (Product # A700-068) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.