Explore
Validate
LearnA700-068-T
antibody from Invitrogen Antibodies
Targeting: TNFRSF18
AITR, CD357, GITR
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A700-068-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GITR/TNFRSF18 Recombinant Rabbit Monoclonal Antibody (BLR068G)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR068G
- Vial size
- 10 µL
- Concentration
- 1000 µg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 µg) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody [BLR057F] (Product # A700-057).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GITR/TNFRSF18 by immunocytochemistry.Sample: FFPE section of RPMI-8226 cells.Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used at 1:200. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4). Counterstain: DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GITR/TNFRSF18 by immunohistochemistry. AntibodySample: FFPE section of tonsil.Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (BLR068G) (A700-068 lot 1) used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GITR/TNFRSF18 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal [BLR068G] (Product #A700-068; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GITR/TNFRSF18 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BLR068G] (Product # A700-068 lot 1) used for IP at 6 µL per reaction. GITR/TNFRSF18 was also immunoprecipitated by rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BL-1066-1D11]. For blotting immunoprecipitated GITR/TNFRSF18 (Product # A700-068) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
