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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [2]
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- Product number
- MA5-21106 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM16 Monoclonal Antibody (5F4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: LDAARRDKEA ELQCTQLDLE RKLKLNENAI SRLQANQKSV LVSVSEVKAV AEMQFGELLA AVRKAQANVM LFLEEKEQAA LSQANGIKAH LEYRSAEMEK SKQELERMA
- Antibody clone number
- 5F4
- Concentration
- 0.31 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRIM16 expressed in transfected 293T cell line by anti-TRIM16 monoclonal antibody (Product # MA5-21106), clone 5F4. Lane 1: TRIM16 transfected lysate (64 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM16 over-expressed 293 cell line, cotransfected with TRIM16 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM16 monoclonal antibody, clone 5F4 (Product # MA5-21106). GAPDH (36.1 kDa) used as specificity and loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TRIM16 using anti-TRIM16 monoclonal antibody (Product # MA5-21106) is approximately 0.03 ng/mL as a capture antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TRIM16 transfected lysate using anti-TRIM16 monoclonal antibody (Product # MA5-21106) and Protein A Magnetic Beads, and immunoblotted with TRIM16 rabbit polyclonal antibody.
