PA5-52804

antibody from Invitrogen Antibodies

Targeting: FGF4 HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52804

Provider

Invitrogen Antibodies

Product name

FGF4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFL Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Gain of FGF4 is a frequent event in KIT/PDGFRA/SDH/RAS-P WT GIST.

Urbini M, Indio V, Tarantino G, Ravegnini G, Angelini S, Nannini M, Saponara M, Santini D, Ceccarelli C, Fiorentino M, Vincenzi B, Fumagalli E, Casali PG, Grignani G, Pession A, Ardizzoni A, Astolfi A, Pantaleo MA
Genes, chromosomes & cancer 2019 Sep;58(9):636-642

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FGF4 in control (vector only transfected HEK293T lysate) and FGF4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a FGF4 Polyclonal Antibody (Product # PA5-52804).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of FGF4 in human testis tissue shows moderate cytoplasmic positivity in cells in seminiferus ducts and leydig cells. Samples were probed using a FGF4 Polyclonal Antibody (Product # PA5-52804).