A300-586A

antibody from Invitrogen Antibodies

Targeting: THAP12 DAP4, P52rIPK, PRKRIR, THAP0

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-586A

Provider

Invitrogen Antibodies

Product name

PRKRIR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of PRKRIR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP; 1/4 of IP reaction loaded) and mouse NIH3T3 (M; 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-PRKRIR antibody BL2255 (Cat. No. A300-586A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PRKRIR was also immunoprecipitated using rabbit anti-PRKRIR antibody BL2254 (Cat. No. A300-585A) at 3 µg/mg lysate. For blotting of immunoprecipitates, ReliaBLOT™ reagents and methods were utilized. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PRKRIR by immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PRKRIR (Cat. No. A300-586A Lot1) used at a dilution of 1:1,000 (0.2µg/ml) and 1:200 (1µg/ml). Detection: DAB.