MA5-27634

antibody from Invitrogen Antibodies

Targeting: SHANK2 CORTBP1, CTTNBP1, ProSAP1, SHANK, SPANK-3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27634

Provider

Invitrogen Antibodies

Product name

SHANK2 Monoclonal Antibody (S23b-6)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S23b-6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SHANK2 in rat brain membrane lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with SHANK2 monoclonal antibody (Product # MA5-27634) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SHANK2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with SHANK2 monoclonal antibody (Product # MA5-27634) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) SHANK2 Antibody, D) Merged image. Magnification: 60x.