H00022933-M01

antibody from Novus Biologicals

Targeting: SIRT2 SIR2L

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00022933-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00022933-M01, RRID:AB_548142

Product name

Mouse Monoclonal Sirtuin 2/SIRT2 Antibody

Antibody type

Monoclonal

Description

IgG purified. SIRT2 - sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia.

García-Berrocoso T, Llombart V, Colàs-Campàs L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A, Bustamante A, Martínez-Saez E, Canals F, Sanchez JC, Montaner J
Molecular & cellular proteomics : MCP 2018 Jan;17(1):175-189

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of SIRT2 over-expressed 293 cell line, cotransfected with SIRT2 Validated Chimera RNAi ( Cat # H00022933-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIRT2 monoclonal antibody (M01), clone 4B11 (Cat # H00022933-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of SIRT2 expression in transfected 293T cell line by SIRT2 monoclonal antibody (M01), clone 4B11.Lane 1: SIRT2 transfected lysate(39.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Detection limit for recombinant GST tagged SIRT2 is approximately 0.1ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of monoclonal antibody to SIRT2 on HeLa cell . Antibody concentration 10 ug/ml.