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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA3-200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIRT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-200 detects human and rat SIRT2 and shows no cross-reactivity to other SIRT isoforms.
- Concentration
- Conc. Not Determined
Submitted references Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.
An Alternatively Spliced Sirtuin 2 Isoform 5 Inhibits Hepatitis B Virus Replication from cccDNA by Repressing Epigenetic Modifications Made by Histone Lysine Methyltransferases.
A role for SIRT2-dependent histone H3K18 deacetylation in bacterial infection.
Adipose tissue-derived cells improve cardiac function following myocardial infarction.
Alcohol-induced alterations in hepatic microtubule dynamics can be explained by impaired histone deacetylase 6 function.
Osthole regulates enzyme protein expression of CYP7A1 and DGAT2 via activation of PPARalpha/gamma in fat milk-induced fatty liver rats.
Menarim BC, El-Sheikh Ali H, Loux SC, Scoggin KE, Kalbfleisch TS, MacLeod JN, Dahlgren LA
Frontiers in immunology 2021;12:734322
Frontiers in immunology 2021;12:734322
An Alternatively Spliced Sirtuin 2 Isoform 5 Inhibits Hepatitis B Virus Replication from cccDNA by Repressing Epigenetic Modifications Made by Histone Lysine Methyltransferases.
Piracha ZZ, Saeed U, Kim J, Kwon H, Chwae YJ, Lee HW, Lim JH, Park S, Shin HJ, Kim K
Journal of virology 2020 Jul 30;94(16)
Journal of virology 2020 Jul 30;94(16)
A role for SIRT2-dependent histone H3K18 deacetylation in bacterial infection.
Eskandarian HA, Impens F, Nahori MA, Soubigou G, Coppée JY, Cossart P, Hamon MA
Science (New York, N.Y.) 2013 Aug 2;341(6145):1238858
Science (New York, N.Y.) 2013 Aug 2;341(6145):1238858
Adipose tissue-derived cells improve cardiac function following myocardial infarction.
Schenke-Layland K, Strem BM, Jordan MC, Deemedio MT, Hedrick MH, Roos KP, Fraser JK, Maclellan WR
The Journal of surgical research 2009 May 15;153(2):217-23
The Journal of surgical research 2009 May 15;153(2):217-23
Alcohol-induced alterations in hepatic microtubule dynamics can be explained by impaired histone deacetylase 6 function.
Shepard BD, Joseph RA, Kannarkat GT, Rutledge TM, Tuma DJ, Tuma PL
Hepatology (Baltimore, Md.) 2008 Nov;48(5):1671-9
Hepatology (Baltimore, Md.) 2008 Nov;48(5):1671-9
Osthole regulates enzyme protein expression of CYP7A1 and DGAT2 via activation of PPARalpha/gamma in fat milk-induced fatty liver rats.
Zhang Y, Xie ML, Xue J, Gu ZL
Journal of Asian natural products research 2008 Jul-Aug;10(7-8):807-12
Journal of Asian natural products research 2008 Jul-Aug;10(7-8):807-12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell, tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Brain (Lane 2), MCF 7 (Lane 3), and HeLa (Lane 4). The blots were probed with Anti-SIRT2 Rabbit Polyclonal Antibody (Product # PA3-200, 1:250 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 30 kDa band corresponding to SIRT2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using Product # PA3-200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using Product # PA3-200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using Product # PA3-200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SIRT2 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SIRT2 Rabbit Polyclonal Antibody (Product # PA3-200) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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