- PA1-914 - Invitrogen Antibodies ATP2B1 antibody

Submitted references Plasma Membrane Ca(2+) ATPase Isoform 4 (PMCA4) Has an Important Role in Numerous Hallmarks of Pancreatic Cancer.

Sritangos P, Pena Alarcon E, James AD, Sultan A, Richardson DA, Bruce JIE
Cancers 2020 Jan 16;12(1)

Histone Deacetylase Inhibitor Treatment Increases the Expression of the Plasma Membrane Ca(2+) Pump PMCA4b and Inhibits the Migration of Melanoma Cells Independent of ERK.

Hegedüs L, Padányi R, Molnár J, Pászty K, Varga K, Kenessey I, Sárközy E, Wolf M, Grusch M, Hegyi Z, Homolya L, Aigner C, Garay T, Hegedüs B, Tímár J, Kállay E, Enyedi Á
Frontiers in oncology 2017;7:95

Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, Boczek T
Frontiers in cellular neuroscience 2017;11:181

The plasma membrane Ca(2+) pump PMCA4b inhibits the migratory and metastatic activity of BRAF mutant melanoma cells.

Hegedũs L, Garay T, Molnár E, Varga K, Bilecz Á, Török S, Padányi R, Pászty K, Wolf M, Grusch M, Kállay E, Döme B, Berger W, Hegedũs B, Enyedi A
International journal of cancer 2017 Jun 15;140(12):2758-2770

Gain-of-function nature of Cav1.4 L-type calcium channels alters firing properties of mouse retinal ganglion cells.

Knoflach D, Schicker K, Glösmann M, Koschak A
Channels (Austin, Tex.) 2015;9(5):298-306

Histone deacetylase inhibitor- and PMA-induced upregulation of PMCA4b enhances Ca2+ clearance from MCF-7 breast cancer cells.

Varga K, Pászty K, Padányi R, Hegedűs L, Brouland JP, Papp B, Enyedi A
Cell calcium 2014 Feb;55(2):78-92

Effect of silencing VDR gene in kidney on renal epithelial calcium transporter proteins and urinary calcium excretion in genetic hypercalciuric stone-forming rats.

Xi QL, Wang SG, Ye ZQ, Zhu ZW, Li C, Bai J, Yu X, Liu JH
Urology 2011 Dec;78(6):1442.e1-7

Adenovirus-delivered microRNA targeting the vitamin D receptor reduces intracellular Ca²⁺ concentrations by regulating the expression of Ca²⁺-transport proteins in renal epithelial cells.

Xi Q, Wang S, Ye Z, Liu J, Yu X, Zhu Z, Su S, Bai J, Li C
BJU international 2011 Apr;107(8):1314-9

Localization of plasma membrane and secretory calcium pumps in the mammary gland.

Faddy HM, Smart CE, Xu R, Lee GY, Kenny PA, Feng M, Rao R, Brown MA, Bissell MJ, Roberts-Thomson SJ, Monteith GR
Biochemical and biophysical research communications 2008 May 9;369(3):977-81

Genetic control of circuit function: Vsx1 and Irx5 transcription factors regulate contrast adaptation in the mouse retina.

Kerschensteiner D, Liu H, Cheng CW, Demas J, Cheng SH, Hui CC, Chow RL, Wong RO
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 5;28(10):2342-52

Differential regulation of the apical plasma membrane Ca(2+) -ATPase by protein kinase A in parotid acinar cells.

Baggaley E, McLarnon S, Demeter I, Varga G, Bruce JI
The Journal of biological chemistry 2007 Dec 28;282(52):37678-93

Plasma membrane calcium pumps in mouse olfactory sensory neurons.

Weeraratne SD, Valentine M, Cusick M, Delay R, Van Houten JL
Chemical senses 2006 Oct;31(8):725-30

The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes.

Sepúlveda MR, Berrocal-Carrillo M, Gasset M, Mata AM
The Journal of biological chemistry 2006 Jan 6;281(1):447-53

Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium.

Talarico EF Jr, Kennedy BG, Marfurt CF, Loeffler KU, Mangini NJ
Molecular vision 2005 Mar 2;11:169-78

Regional distribution of Na,K-ATPase activity in porcine lens epithelium.

Tamiya S, Dean WL, Paterson CA, Delamere NA
Investigative ophthalmology & visual science 2003 Oct;44(10):4395-9

Regional distribution of Na,K-ATPase activity in porcine lens epithelium.

Tamiya S, Dean WL, Paterson CA, Delamere NA
Investigative ophthalmology & visual science 2003 Oct;44(10):4395-9

Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies.

Caride AJ, Filoteo AG, Enyedi A, Verma AK, Penniston JT
The Biochemical journal 1996 May 15;316 ( Pt 1):353-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase (Product # PA1-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase (Product # PA1-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Combination treatment with the mutant BRAF inhibitor vemurafenib and histone deacetylase inhibitors (HDACi) . BRAF wild-type MEWO and BRAF-mutant A375 and A2058 cells were treated with the mutant BRAF inhibitor vemurafenib (0.5 uM), HDACi SAHA (1 uM), and valproate (2 mM) alone or in combination for 48 h. After Western blotting expression of PMCA4b, PMCA1, SERCA2, SERCA3, pERK, and ERK proteins were analyzed. As a positive control of PMCA1 and PMCA4b, total cell lysate of COS-7 cells was used, which endogenously express both proteins ( 9 ). (A) The expression of PMCA proteins was detected with isoform-specific and pan-PMCA antibodies. (B) After densitometric analysis, changes in PMCA4b (B) , ERK (C) and SERCA2 (D) protein levels were normalized to the expression levels of beta-tubulin and expressed as fold increase over the untreated controls. Bars represent mean +- SE from three independent experiments. Significance compared to control is denoted by asterisks (* p < 0.05), using two-tailed paired t -test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 Effect of silencing VDR gene expression on Ca 2+ -transport proteins expression levels in NRK cells. Western blot for VDR, TRPV5, TRPV6, calbindin-D 28K , PMCA1b, and NCX1 from AdmiVDR- and Negative-vector infected NRK cells at different time points.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Expression of PMCA isoforms in multiple pancreatic cell lines. ( A ) Representative Western immunoblot showing the relative protein expression of total/pan-PMCA and PMCA isoform 1-4 in pancreatic cancer (MIA PaCa-2 and PANC-1) and non-malignant pancreatic cells (human pancreatic ductal epithelial (HPDE) and human pancreatic stellate cells (hPSC)). Mouse brain lysate was used as a positive control for PMCA expressions and beta-Actin was used as a protein loading control. ( B ) PMCA4 protein expression in each cell line was quantified from Western blot bands and normalized to beta-Actin housekeeping protein. ( C ) The relative expressions of ATP2B1-4 (PMCA1-4 mRNA) in each cell line were quantified by RT-qPCR. Data are expressed as relative mRNA expression normalized to corresponding S18 rRNA controls (2 -DeltaCtau ). Statistical comparisons were made using the Kruskal-Wallis test with Dunn's multiple comparison test and two-way analysis of variance (ANOVA) with Dunnett's multiple comparison test. Data are expressed as mean +- SEM. (n = 4-5, 4 replicates per treatment condition). * represents statistical significance where p < 0.05.

PA1-914

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