Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 631101 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#631101, RRID:AB_2138144
- Product name
- Purified anti-Microtubule Associated Protein 2ab (MAP2ab)
- Antibody type
- Monoclonal
- Antigen
- Microtubule protein enriched for kinesin
- Description
- Microtubule associated protein 2 also know as MAP2, has three isoforms that include two high molecular weight forms MAP2A and MAP2B (~280KD), and a low molecular weight form MAB2C (~70KD). MAP2 belongs to the microtubule-associated protein family and expressed in the nervous system. The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. MAP2 has been shown to interact with Fyn, Grb2, PRKACA, and CPEB1. The MT-08 antibody has been shown to be useful for Western blotting, Immunoprecipitation, Immunohistochemistry (frozen sections), Immunocytochemistry, and ELISA of the human, mouse and porcine MAP2A and MAP2B isoforms.
- Reactivity
- Human, Mouse, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4° C.
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Mouse brain tissue extract was resolved by electrophoresis, transferred nitrocellulose, and probed with monoclonal anti-MAP2ab (clone MT-08) antibody. Proteins were visualized using HRP goat anti-mouse IgG and a chemiluminescence detection system.
- Conjugate
- Unconjugated
