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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- NBP1-92711 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92711, RRID:AB_11007609
- Product name
- Mouse Monoclonal MAP2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Note that since the epitope for this antibody in within the projection domain found only in MAP2A and MAP2B, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.
- Reactivity
- Human, Mouse, Rat, Bovine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cytoplasmic sequestration of FUS/TLS associated with ALS alters histone marks through loss of nuclear protein arginine methyltransferase 1.
Synaptic activity bidirectionally regulates a novel sequence-specific S-Q phosphoproteome in neurons.
Enrichment and characterization of human dermal stem/progenitor cells by intracellular granularity.
Tibshirani M, Tradewell ML, Mattina KR, Minotti S, Yang W, Zhou H, Strong MJ, Hayward LJ, Durham HD
Human molecular genetics 2015 Feb 1;24(3):773-86
Human molecular genetics 2015 Feb 1;24(3):773-86
Synaptic activity bidirectionally regulates a novel sequence-specific S-Q phosphoproteome in neurons.
Siddoway B, Hou H, Yang H, Petralia R, Xia H
Journal of neurochemistry 2014 Mar;128(6):841-51
Journal of neurochemistry 2014 Mar;128(6):841-51
Enrichment and characterization of human dermal stem/progenitor cells by intracellular granularity.
Shim JH, Lee TR, Shin DW
Stem cells and development 2013 Apr 15;22(8):1264-74
Stem cells and development 2013 Apr 15;22(8):1264-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of different tissue lysates using mouse mAb to MAP2A/B (5H11), NBP1-92711, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain lysate. A band observed at about 280 kDa corresponds to full length MAP2a and MAP2B protein. MAP2A/B is expressed heavily in brain particularly in cortical regions, but is a more minor component of spinal cord. Note that the epitope for this antibody in within the projection domain, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry Free-Floating: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-92711 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.
