Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX114593 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114593, RRID:AB_10619335
- Product name
- Nucleobindin 1 antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Huang HJ, Lin CC, Chou HC, Chen YW, Lin ST, Lin YC, Lin DY, Lyu KW, Chan HL
Molecular bioSystems 2014 Dec;10(12):3086-100
Molecular bioSystems 2014 Dec;10(12):3086-100
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX114593 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE GTX114593 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Nucleobindin 1(GTX114593) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody [N3C3] (GTX114593) diluted at 1:500.Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.