HPA012612

antibody from Atlas Antibodies

Targeting: CADM4 IGSF4C, Necl-4, SynCAM4, TSLL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA012612

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA012612, RRID:AB_1851530

Product name

Anti-CADM4

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GGIIICEAQNQALPSGHSKQTQYVLDVQYSPTARI
HASQAVVREGDTLVLTCAVTGNPRPNQIRWNRGNE
SLPERAEAVGETLTLPGLVSADNGTYTCEASNKHG
HARALYVLVVYDPGAVVEAQTSVPY


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Sample type

MOUSE, RAT

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-CADM4 antibody. Corresponding CADM4 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN