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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-118201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of PSMA2 in Lane A: H1299 Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lane D: Jurkat Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-118201) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 25 kDa, Observed band size: 25 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PSMA2 Polyclonal Antibody (Product # PA5-118201) at 1:500 dilution. Lane A: H1299 Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human PSMA2 in human liver with PSMA2 Polyclonal Antibody (Product # PA5-118201, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PSMA2 in Lane A: 0.5 mg A431 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118201) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 26 kDa , Observed band size : 30 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSMA2 Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 µL with PSMA2 Polyclonal Antibody (Product # PA5-118201) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PSMA2 Polyclonal Antibody (Product # PA5-118201), at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 30 kDa.
