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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-1000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNAI1/GNAI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-1000 detects G protein alpha inhibitor 1/2 from human, rat, and mouse tissues and cells.
- Concentration
- 1 mg/mL
Submitted references Differential effects of antidepressants escitalopram versus lithium on Gs alpha membrane relocalization.
Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1.
Donati RJ, Schappi J, Czysz AH, Jackson A, Rasenick MM
BMC neuroscience 2015 Jul 11;16:40
BMC neuroscience 2015 Jul 11;16:40
Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1.
Jang HJ, Kokrashvili Z, Theodorakis MJ, Carlson OD, Kim BJ, Zhou J, Kim HH, Xu X, Chan SL, Juhaszova M, Bernier M, Mosinger B, Margolskee RF, Egan JM
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 18;104(38):15069-74
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 18;104(38):15069-74
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of G Protein alpha Inhibitor 1 & 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GNAI1/GNAI2 Rabbit Polyclonal Antibody (Product # PA1-1000) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Gialpha membrane compartmentalization is not altered by chronic antidepressant or lithium treatment. Purified lipid rafts isolated using sucrose gradient flotation revealed that neither chronic antidepressant treatment nor chronic lithium treatment move Gialpha out of lipid rafts. a The figure shows the percentage of change in Gialpha protein in the lipid raft membrane fractions compared to control (n = 4 for control, escitalopram, and lithium; n = 3 for valproate). Data were analyzed by one-way ANOVA followed by Tukey's multiple comparison test for post hoc comparison of means. Data are represented as mean +- SEM b A representative immunoblot of lipid raft Gsalpha from the various treatment paradigms as explained in "" Methods "". C control, E escitalopram, L lithium, V valproic acid.
