NBP1-82964

antibody from Novus Biologicals

Targeting: SV2A KIAA0736, SV2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-82964

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-82964, RRID:AB_11035437

Product name

Rabbit Polyclonal SV2A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human SV2A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Amyloid beta a4 precursor protein-binding family B member 1 (FE65) interactomics revealed synaptic vesicle glycoprotein 2A (SV2A) and sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) as new binding proteins in the human brain.

Nensa FM, Neumann MH, Schrötter A, Przyborski A, Mastalski T, Susdalzew S, Looβe C, Helling S, El Magraoui F, Erdmann R, Meyer HE, Uszkoreit J, Eisenacher M, Suh J, Guénette SY, Röhner N, Kögel D, Theiss C, Marcus K, Müller T
Molecular & cellular proteomics : MCP 2014 Feb;13(2):475-88

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SV2A Antibody [NBP1-82964] - Analysis of SV2A in (1) undifferentiated SH-SY5Y, (2) differentiated SH-SY5Y (neurons) and (3) mouse brain using anti-SV2A antibody. 30ug of protein/lane. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SV2A Antibody [NBP1-82964] - Analysis in human cell lines U2OS and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SV2A Antibody [NBP1-82964] - Analysis in human cerebral cortex tissue.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: SV2A Antibody [NBP1-82964] - Staining of neuroblastoma cells SH-SY5Y differentiated into neurons. Lane (1) 10ug of input, 500ug used for IP. 10ul of antibody used for incubation with lysate (overnight incubation at 4C with rotation) prior to addition of beads. Image from verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Analysis in human cerebral cortex and small intestine tissues using HPA007863 antibody. Corresponding SV2A RNA-seq dat

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human small intestine shows no positivity in glandular cells.