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LearnH00009114-M01
antibody from Novus Biologicals
Targeting: ATP6V0D1
ATP6D, ATP6DV, P39, VATX, Vma6, VPATPD
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00009114-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009114-M01, RRID:AB_537014
- Product name
- Mouse Monoclonal ATP6V0D1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ATP6V0D1 - ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Proteomic analysis of endosomes from genetically modified p14/MP1 mouse embryonic fibroblasts.
Stasyk T, Holzmann J, Stumberger S, Ebner HL, Hess MW, Bonn GK, Mechtler K, Huber LA
Proteomics 2010 Nov;10(22):4117-27
Proteomics 2010 Nov;10(22):4117-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP6V0D1 Antibody (2G12) [H00009114-M01] - Analysis of ATP6V0D1 expression in transfected 293T cell line by ATP6V0D1 monoclonal antibody (M01), clone 2G12.Lane 1: ATP6V0D1 transfected lysate(40.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP6V0D1 Antibody (2G12) [H00009114-M01] - ATP6V0D1 monoclonal antibody (M01), clone 2G12 Analysis of ATP6V0D1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ATP6V0D1 Antibody (2G12) [H00009114-M01] - Detection limit for recombinant GST tagged ATP6V0D1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATP6V0D1 Antibody (2G12) [H00009114-M01] - Analysis of monoclonal antibody to ATP6V0D1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 0.5 ug/ml.
