Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3117 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IGSF8/CD316 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IGSF8/CD316 in direct ELISAs. Detects human, mouse, and rat IGSF8/CD316 in Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references EWI-2 negatively regulates TGF-β signaling leading to altered melanoma growth and metastasis.
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
IgSF8: a developmentally and functionally regulated cell adhesion molecule in olfactory sensory neuron axons and synapses.
Wang HX, Sharma C, Knoblich K, Granter SR, Hemler ME
Cell research 2015 Mar;25(3):370-85
Cell research 2015 Mar;25(3):370-85
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
Bye CR, Jönsson ME, Björklund A, Parish CL, Thompson LH
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 14;112(15):E1946-55
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 14;112(15):E1946-55
IgSF8: a developmentally and functionally regulated cell adhesion molecule in olfactory sensory neuron axons and synapses.
Ray A, Treloar HB
Molecular and cellular neurosciences 2012 Jul;50(3-4):238-49
Molecular and cellular neurosciences 2012 Jul;50(3-4):238-49
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat IGSF8/CD316 by Simple WesternTM. Detection of Human, Mouse, and Rat IGSF8/CD316 by Simple WesternTM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat IGSF8/CD316 by Western Blot. Detection of Rat IGSF8/CD316 by Western Blot.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse IGSF8/CD316 by Western Blot. Detection of Human and Mouse IGSF8/CD316 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IGSF8/CD316 in Neuro-2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
