PA5-47450

antibody from Invitrogen Antibodies

Targeting: IGSF8 CD316, CD81P3, EWI2, PGRL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47450

Provider

Invitrogen Antibodies

Product name

IGSF8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IGSF8 in SH‚SY5Y human neuroblastoma cell line, DU145 human prostate carcinoma cell line, and bEnd.3 mouse endothelioma cell line. Samples were incubated in IGSF8 polyclonal antibody (Product # PA5-47450) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for IGSF8/CD316 at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IGSF8 in rat brain (hippocampus) tissue. Samples were incubated in IGSF8 polyclonal antibody (Product # PA5-47450) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for IGSF8/CD316 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IGSF8 in 0.2 mg/mL lysates of human cerebellum tissue, human hippocampus tissue, Neuro‚2A mouse neuroblastoma cell line, and rat hippocampus tissue. Samples were incubated in IGSF8 polyclonal antibody (Product # PA5-47450) using a dilution of 20 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 1:50. A specific band was detected for IGSF8/CD316 at approximately 71-80 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of IGSF8 in immersion fixed Neuro‚2A mouse neuroblastoma cell line. Samples were incubated in IGSF8 polyclonal antibody (Product # PA5-47450) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of IGSF8 in Neuro‚2A mouse neuroblastoma cell line. Samples were incubated in IGSF8 polyclonal antibody (Product # PA5-47450) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.