- PA1-742 - Invitrogen Antibodies STXBP1 antibody

Submitted references Congenital Anophthalmia and Binocular Neonatal Enucleation Differently Affect the Proteome of Primary and Secondary Visual Cortices in Mice.

Laramée ME, Smolders K, Hu TT, Bronchti G, Boire D, Arckens L
PloS one 2016;11(7):e0159320

The Toll-like receptor-3 agonist polyinosinic:polycytidylic acid triggers nigrostriatal dopaminergic degeneration.

Deleidi M, Hallett PJ, Koprich JB, Chung CY, Isacson O
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Dec 1;30(48):16091-101

Apical targeting of syntaxin 3 is essential for epithelial cell polarity.

Sharma N, Low SH, Misra S, Pallavi B, Weimbs T
The Journal of cell biology 2006 Jun 19;173(6):937-48

Munc18c regulates insulin-stimulated glut4 translocation to the transverse tubules in skeletal muscle.

Khan AH, Thurmond DC, Yang C, Ceresa BP, Sigmund CD, Pessin JE
The Journal of biological chemistry 2001 Feb 9;276(6):4063-9

A novel ubiquitous form of Munc-18 interacts with multiple syntaxins. Use of the yeast two-hybrid system to study interactions between proteins involved in membrane traffic.

Hata Y, Südhof TC
The Journal of biological chemistry 1995 Jun 2;270(22):13022-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Munc-18 in rat brain homogenate immunoprecipitated with Product # PA1-742. 1. Extract only. 2-4. 750 µg of extract immunoprecipitated with 1 ug, 5 ug, and 10 µg of Product # PA1-742, respectively.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extract of mouse brain (30 µg lysate) (Lane 1). The blots were probed with Anti-MUNC18 Rabbit Polyclonal Antibody (Product # PA1-742, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 67 kDa band corresponding to MUNC18 was observed in the tissue extract tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Rat Liver (Lane 3) and Rat Kidney (Lane 4). The blot was probed with Anti- MUNC18 Rabbit Polyclonal Antibody (Product # PA1-742, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 67 kDa band corresponding to MUNC18 was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be MUNC18 negative.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of MUNC18 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUNC18 Rabbit Polyclonal Antibody (Product # PA1-742) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of MUNC18 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUNC18 Rabbit Polyclonal Antibody (Product # PA1-742) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of MUNC18 was done on U-87 MG cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with MUNC18 Rabbit Polyclonal Antibody (PA1-742, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 2 Proteins involved in synaptic transmission. The expression of glutamine synthetase (GLS), glutamate AMPA 2 (GluA2), syntaxin binding protein 1 (STXBP1), glutamate vesicular transporter 1 (vGluT2) and 2 (vGluT2), glutamate decarboxylase 65 kb (GAD65) and 67 kb (GAD67), synapsin 2a (Syn2a) and 2b (Syn2b) were analyzed by Western blotting. White bars are controls, grey bars are enucleated and black bars are anophthalmic mice. Results from V1 (left), V2M (middle) and V2L (right) are shown for each protein. ** P < 0.01.

PA1-742

Antibody data