11459-1-AP

antibody from Invitrogen Antibodies

Targeting: STXBP1 hUNC18, MUNC18-1, rbSec1, UNC18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11459-1-AP

Provider

Invitrogen Antibodies

Product name

STXBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: HELTFQAMS YDLLPIENDV YKYETSGIGE ARVKEVLLDE DDDLWIALRH KHIAEVSQEV TRSLKDFSSS KRMNTGEKTT MRDLSQMLKK MPQYQKELSK YSTHLHLAED CMKHYQGTVD KLCRVEQDLA MGTDAEGEKI KDPMRAIVPI LLDANVSTYD KIRIILLYIF LKNGITEENL NKLIQHAQIP PEDSEIITNM AHLGVPIVTD STLRRRSKPE RKERISEQTY QLSRWTPIIK DIMEDTIEDK LDTKHYPYIS TRSSASFSTT AVSARYGHWH KNKAPGEYRS GPRLIIFILG GVSLNEMRCA YEVTQANGKW EVLIGSTHIL TPQKLLDTLK KLNKTDEEIS S (245-594 aa encoded by BC015749)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.46 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A375 cells using 11459-1-AP (STXBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-STXBP1 (IP:11459-1-AP, 4ug; Detection:11459-1-AP 1:1000) with HeLa cells lysate 2400ug.