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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 101-M716 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- Emilin-2
- Antibody type
- Monoclonal
- Antigen
- Recombinant gC1q domain of EMILIN2
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- (#828B3B3)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis with anti-human Emilin-2. 20ng of recombinant human Emilin2 was loaded, 5µg/ml of anti-human Emilin2 was used (ECL, 45 sec exposition).
- Sample type
- Purified recombinant protein
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human Emilin-2 (green) in human colon-rectal tissue(frozen section). Nuclear staining with TO-PRO3 in blue. Scale bar: 37.00 μm.
